medical microbiology [serology] (part III)

Thyroglobulin & Thyroid Microsomal Antibodies (THYMA)

It is to test for presence of thyroidic autoantibodies, where the Ab attacks the thyroid. 2 tests are usually done, thyroglobulin antibodies test (ATG) and thyroid microsomal antiboides test (AMC). these 2 tests are usually ordered together. Presence of either Ab means that the person has autoAb to his/her thyroid.

The principle is agglutination and the steps are almost the same with MPA, only the volume of sample diluent and serum are different.

For both tests, the first and second well is added with 50ul of sample diluent and 75ul for 3-4th well for control and 3-6th well for each sample. Then, 10ul of control is added to the 1st well of control. Same goes to each specimen. After that, a 25 serial dilution is done down the row till the 4th well for control and 6th well for each specimen.

Same with MPA, 1 drop of unsensitised particles is added to second well and 1 drop of sensitised particles to 3 wells onwards. The sides of the plates are then patted and incubate overnight at room temperature.

The results is same with MPA, where unsensitised cells and negative results will show button in the center of the well, while positive results will show agglutination and absence of button in the center of well.

yaNLing xD