Mycoplasma pneumoniae antibodies
if one is infected by M. pneumoniae, one will produce Ab against it, thus in this test, it is to see if the patient had been infected with the bacteria.
The principle is this test is also agglutination, where the results are similar to the results of TPHA i mentioned earlier.. if the result is negative, the sensitised particles will settle to the bottom of the well to form a compact ring or button.
the steps is more simpler here. 1st, add 100ul of sample diluent into 1st well, and add 25ul of sample diluent into 2nd to 4th well for control and 2nd to 6th well for each patient's serum. Then add 25ul of serum into 1st well and do a 25ul serial dilution down the row. Next, add i drop of unsensitised particle into 2nd well and add 1 drop of sensitised particles into the 3rd and 4th well for control and 3rd to 6th well for specimens. Pat the sides of the plate and incubate for 3 hours or overnight.
it is important that the 2nd well must be negative results, as the unsensitised particles are not coated with the bacteria Ag, thus there will not be any agglutination. If there is positive results for 2nd well, it might be due to cross contamination or added wrong reagent.
the results of each specimen are then recorded and report in terms of the titre as follow: 1:40(3rd well), 1:80(4th well), 1:160(5th well), 1:320(6th well) and >1:320(when there is still agglutination at 6th well).
yaNLing xD
*will add more tests soon when im free~!*
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