Hi, there! Muna here XD
SIP is finally over. 5 months of hard work, blood (literally!), sweat and tears.
Good luck to all for the MP project reports.
Streptex is a rapid latex test used to determine the Lancefield grouping of streptococci. This test uses the principle of antigen-antibody binding. The antigen-antibody complex forms as a visible precipitate in a positive reaction.
Most of the Streptococcus sp. contain group-specific antigen. These antigens are actually carbohydrate structural components of the bacterial cell wall. It was shown by Lancefield that these antigens can be extracted in a soluble form and tested using precipitation reactions with homologous antisera.
In Streptex, the antigen extract is obtained using a simple enzyme extraction procedure. Polystyrene latex coated particles coated with group-specific antibodies are used to identify the antigen in the extract. Agglutination will occur strongly in the presence of the specific homologous antigen and remain smooth or milky in its absence.
The latex reagent contains a combination of fibrinogen, IgG and anti-capsular monoclonal antibodies to allow recognition of capsular polysaccharides of Staphylococcus aureus.
5 pure growth colonies of Streptococcus sp. are used to make a 0.5 McFarland suspension in 400ml of Extraction Enzyme.
Incubate at 35oC between 10 mins and 1 hour.
Re-suspend the test kit latex by shaking.
Add 1 drop of each test kit latex (A, B, C, D, F, G) to the test circles on the reaction card.
Add 1 drop of extracted sample to each test circle.
Rock card horizontally or use a disposable rod to mix for 1 minute.
Read the result immediately after 1 minute of shaking.
Positive
Visible clumps
Negative
No clumping/agglutination
Milky appearance
The main use of this test is to test for the more virulent beta-haemolytic strains of Streptococcus.
*Colonies should be taken from Blood Agar Plates (BAP), Chocolate Agar (CHOC) or CAN (Columbia agar) and not be more than 48 hours of growth.
References:
Taken from
http://www.remelinc.com/Clinical/DiagnosticTests/Streptex.aspx
http://www.dcss.cs.amedd.army.mil/field/FLIP%20Disk%2041/.\Documents\M403_Micro_Aug\Streptex.DOC
on 21 Sep 2009
Most of the Streptococcus sp. contain group-specific antigen. These antigens are actually carbohydrate structural components of the bacterial cell wall. It was shown by Lancefield that these antigens can be extracted in a soluble form and tested using precipitation reactions with homologous antisera.
In Streptex, the antigen extract is obtained using a simple enzyme extraction procedure. Polystyrene latex coated particles coated with group-specific antibodies are used to identify the antigen in the extract. Agglutination will occur strongly in the presence of the specific homologous antigen and remain smooth or milky in its absence.
The latex reagent contains a combination of fibrinogen, IgG and anti-capsular monoclonal antibodies to allow recognition of capsular polysaccharides of Staphylococcus aureus.
5 pure growth colonies of Streptococcus sp. are used to make a 0.5 McFarland suspension in 400ml of Extraction Enzyme.
Incubate at 35oC between 10 mins and 1 hour.
Re-suspend the test kit latex by shaking.
Add 1 drop of each test kit latex (A, B, C, D, F, G) to the test circles on the reaction card.
Add 1 drop of extracted sample to each test circle.
Rock card horizontally or use a disposable rod to mix for 1 minute.
Read the result immediately after 1 minute of shaking.
Positive
Visible clumps
Negative
No clumping/agglutination
Milky appearance
The main use of this test is to test for the more virulent beta-haemolytic strains of Streptococcus.
*Colonies should be taken from Blood Agar Plates (BAP), Chocolate Agar (CHOC) or CAN (Columbia agar) and not be more than 48 hours of growth.
References:
Taken from
http://www.remelinc.com/Clinical/DiagnosticTests/Streptex.aspx
http://www.dcss.cs.amedd.army.mil/field/FLIP%20Disk%2041/.\Documents\M403_Micro_Aug\Streptex.DOC
on 21 Sep 2009
Muna
0703791D
hello muna.. yes sip is over.. and mp is soon over too... tomor..
ReplyDeleteanyways.. y the pure colonies cannot be more than 48 hours of growth? is it that it will give false grping results?
thanks in advance~!
yaNLing xD
This comment has been removed by the author.
ReplyDeleteSome of its characteristics may fade with time and maturity (old age) of the colonies and thus be inaccurate...
ReplyDeleteMuna
0703791D
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